A thymoma can be suspected in a patient with myasthenia gravis or one of the other autoimmune or endocrine disorders noted above. Imaging • A thymoma can be strongly suspected if a mass is seen on a chest x-ray in the location of the thymus in the center of the chest. Endoscopy and Biopsy • The biopsy needed for a definite diagnosis may sometimes be obtainable with a fine needle aspiration (FNA) but may also require definitive surgery.